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报道了用双波长薄层层析扫描法测定泰必利血药及尿药浓度。该法准确性及重现性较高。经提取和浓缩的血样或尿样于硅胶60GF~(254)薄层板上,展开剂展开后,在254nm紫外灯下观察斑点。泰必利及内标达可罗宁的R_f值分别为0.28、0.34;泰必利相对于内标的比移值R(st)为0.82。根据斑点面积用内标法计算血样或尿样浓度。血清中泰必利的最低检测浓度为0.5μg/ml,尿液中0.4μg/ml;测定血样及尿样的回收率分别为102.6％、100.7％;日内和日间CV％均小于11。测得该药在正常成人尿中原药排泄为82.28％,CV％为5.04。
The use of dual-wavelength thin-layer chromatography scanning for the determination of thiazole blood and urine concentrations was reported. The method is highly accurate and reproducible. The extracted and concentrated blood or urine sample was applied to a silica gel 60GF-254 thin plate. After the developing solution was developed, the spots were observed under a 254 nm UV lamp. The R_f values of tiapride and the internal standard Dacronin were 0.28 and 0.34, respectively; the titer (R) of tipiro relative to the internal standard was 0.82. The blood sample or urine sample concentration was calculated according to the spot area using the internal standard method. The lowest detection concentration of Tiapride in serum was 0.5 μg/ml, and 0.4 μg/ml in urine. The recoveries of blood samples and urine samples were 102.6% and 100.7%, respectively; and the intraday and interday CV% were both less than 11. The excretion of the drug in normal adult urine was 82.28%, and the CV% was 5.04.