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目的 研究杀伤细胞免疫球蛋白样受体(KIR)与人类白细胞抗原(HLA)Ⅰ类在云南独龙族人群中的基因频率及配对分布。方法 从云南独龙族无偿献血人群中选取157名健康无关志愿者,采用聚合酶链式反应-序列特异性引物扩增(PCR-SSP)进行KIR基因检测;采用聚合酶链式反应-直接测序分型法(PCR-SBT)进行HLA-A、B、C等位基因分型。KIR基因携带者频率和HLA-A,-B和-C等位基因频率以直接计数法计算。结果 在所有检测的样本中,HLA-C1和HLA-C2频率分别为92.4%和7.6%。含有Bw4基序的HLA-A、-B基因频率分别为5.1%和6.4%。所有个体均含有抑制性KIR/HLA-C配受体对,含有1、2、3对KIR/HLA-C配受体对的频率分别为87.2%、8.3和4.5%。在KIR/HLA-Bw4配对中,KIR3DL1+HLA-Bw4配受体对频率为12.1%,KIR3DS1+HLA-Bw4配受体对频率为1.3%,2者均含有的频率为5.1%,2者均没有的频率为81.5%。结论 云南独龙族人群HLA及KIR/HLA配受体对有独特的分布特征,抑制性的KIR-HLA配受体对占主导地位。
Objective To study the frequency and distribution of killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) Ⅰ in Dulong nationality in Yunnan. Methods A total of 157 healthy unrelated volunteers were recruited from Yunnan Dulong tribe donated by blood donors. PCR-SSP was used to detect KIR gene. Polymerase chain reaction-direct sequencing Type method (PCR-SBT) HLA-A, B, C allele type. KIR gene carrier frequency and HLA-A, -B, and -C allele frequencies were calculated by direct counting. Results Of all the samples tested, HLA-C1 and HLA-C2 frequencies were 92.4% and 7.6%, respectively. The frequencies of HLA-A and B genes containing Bw4 motif were 5.1% and 6.4%, respectively. All individuals contained inhibitory KIR / HLA-C receptor pairs, with frequencies of 1, 2, 3 pairs of KIR / HLA-C receptor partners being 87.2%, 8.3 and 4.5%, respectively. In the KIR / HLA-Bw4 pairing, the frequency of KIR3DL1 + HLA-Bw4 receptor pair was 12.1%, the frequency of KIR3DS1 + HLA-Bw4 receptor partner was 1.3%, and both contained 5.1%, both of which No frequency is 81.5%. Conclusion The distribution of HLA and KIR / HLA ligands in Dulong nationality in Yunnan Province is unique, and the dominant KIR-HLA ligands are predominant.