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采用４－二甲基偶氮苯４－磺酰氯（ＤＡＢＳ－ｃｌ）色标试剂柱前标记，经反相高压液相色谱（ＲＰ－ＨＰＬＣ）对急性非淋巴细胞白血病（ＡＮＬＬ）患儿血液氨基酸进行分析，并与正常儿进行对照，结果：①ＡＮＬＬ患儿血液氨基酸中谷氨酸（ＧＩｕ），甘氨酸（Ｇｌｙ），苯丙氨酸（Ｐｈｅ），色氨酸（Ｔｒｐ），亮氨酸（Ｌｅｕ），水平明显高于正常。而丙氨酸（Ａｌａ），苏氨酸（Ｔｈｒ），组氨酸（Ｈｉｓ）水平低于正常；②ＡＮＬＬ患者治疗前后比较：治疗后甘氨酸、苯氨酸、色氨酸水平明显降低，并与正常水平接近。而组氨酸、丙氨酸水平明显升高，也接近于正常；③不同预后组比较：苯丙氨酸、色氨酸水平在预后差组明显高于预后好组，而组氨酸水平则低于预后好组。结果表明，ＡＮＬＬ患者存在明显氨基酸代谢异常。
The amino acids in blood of children with acute non-lymphocytic leukemia (ANLL) were analyzed by reverse-phase high-pressure liquid chromatography (RP-HPLC) with the pre-column labeling of 4-dimethylazobenzene 4-sulfonyl chloride The results were as follows: (1) Glutamic acid (GIu), glycine (Gly), phenylalanine (Phe), tryptophan (Trp), leucine (Leu) , The level was significantly higher than normal. While the levels of Ala, Thr and His were lower than normal. ②The comparison of ANLL patients before and after treatment: The levels of glycine, methionine and tryptophan decreased significantly after treatment, Close to level. While the levels of histidine and alanine increased significantly, which were also close to normal. ③Compared with the different prognosis groups, the levels of phenylalanine and tryptophan in the poor prognosis group were significantly higher than those in the good prognosis group, while the levels of histidine Lower than the prognosis group. The results showed that patients with ANLL obvious abnormalities of amino acid metabolism.