论文部分内容阅读
目的了解芜湖市手足口病肠道病毒感染情况及型别,为防控提供依据并对标本的核酸检测结果进行评价。方法采集2013年芜湖市手足口病标本490人份,提取病毒RNA,采用real-time RT-PCR方法对病毒RNA进行EV、EV71和Cox A16特异性核酸检测,并从不同地区、年龄、性别、月份、病例类型对结果进行分析。结果 490份标本中EV核酸阳性333例,总阳性率为68.0%。333份阳性标本中,EV71型阳性184份,占55.3%;Cox A16型阳性44人,占13.2%;其他肠道病毒阳性105人,占31.5%。阳性病原检出高峰在4~7月份,共检出218份,占65.5%;次高峰在11~12月份,共检出72份,占21.6%。0~5岁儿童EV阳性病例占95.5%。EV阳性病例中,散发病例为主,占85.3%,聚集性病例占14.7%,散发病例和聚集病例EV71、Cox A16、未分型肠道病毒构成差异无统计学意义(P>0.05);农村病例占54.1%,城镇病例占45.9%,农村与城镇病例EV71、Cox A16、未分型肠道病毒构成差异无统计学意义(P>0.05)。结论 2013年芜湖地区手足口病的流行株以EV71型为主,0~5岁儿童为高危人群,监测重点时间在4~7月份和11~12月份。荧光定量RT-PCR可快速诊断手足口病病原。
Objective To understand the status and types of enterovirus infection in hand, foot and mouth disease in Wuhu City, and to provide basis for prevention and control and to evaluate the results of nucleic acid detection of specimens. Methods A total of 490 human hand-foot-and-mouth disease samples were collected from Wuhu city in 2013. The viral RNA was extracted and the specific RNAs of EV, EV71 and Cox A16 were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) Month, case type analysis of the results. Results Among the 490 specimens, 333 were positive for EV nucleic acid with a total positive rate of 68.0%. Of 333 positive samples, 184 were positive for EV71, accounting for 55.3%; 44 were positive for Cox A16, accounting for 13.2%; and 105 were other positive for enterovirus, accounting for 31.5%. Positive pathogens detected peak in April-July, 218 were detected, accounting for 65.5%; sub-peak in 11 to December, a total of 72 were detected, accounting for 21.6%. EV-positive children aged 0 ~ 5 years accounted for 95.5%. Among the EV positive cases, sporadic cases accounted for 85.3% and clustered cases accounted for 14.7%. There were no significant differences in the distribution of EV71, Cox A16 and undifferentiated enterovirus between sporadic cases and aggregated cases (P> 0.05); rural The cases accounted for 54.1% and the cases in urban areas accounted for 45.9%. There was no significant difference in the composition of EV71, Cox A16 and unsorted enterovirus between rural and urban areas (P> 0.05). Conclusion The prevalence of HFMD in Wuhu in 2013 was mainly in EV71 type. Children aged 0-5 years were at high risk. The monitoring time was between April and July and from November to December. Fluorescent quantitative RT-PCR can be rapid diagnosis of hand, foot and mouth disease pathogens.