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硒与2,3一二氮基蔡在。.1N盐酸介质中,于沸水浴中加热5分钟,形成整合物.用EDTA掩蔽干扰离子,以环己烷萃取,将有机相注入岛津LC-3A高效液相色谱仪色谱柱中,用RF-51oLC荧光检测器555nm处(374nm激发)侧fX荧光强度,}IC-RIB处理机记录峰面积.对硒与DAN的赘合萃取及高效液相色谱条件进行了试验:沸水浴加热3-5分钟为宜。环己烷萃取2 -10分钟;相比1:9结果一致.赘合物至少稳定24小时.上述操作应在暗处进行,试液应避光保存.高效液相色谱法采用Zorbax ODS 5协柱,乙腊为流动相.保留时间为5.47分。有效塔板数为1L628.检出限为0.1a6.标准曲线的线性范围为2,., gng.试样5次测定的相对标准偏差为8.82％.w
Selenium with a 2,3-dinitro Cai in. .1N hydrochloric acid medium and heated in a boiling water bath for 5 minutes to form a complex. The interference ions were masked with EDTA, extracted with cyclohexane, and the organic phase was injected into a Shimadzu LC-3A high performance liquid chromatography column with an RF -51oLC fluorescence detector 555nm (374nm excitation) side of the fX fluorescence intensity,} IC-RIB processor recording peak area. Selenium and DAN of the combined extraction and high performance liquid chromatography conditions were tested: heated boiling water bath 3-5 Minute is appropriate. Cyclohexane extraction 2 -10 minutes; 1: 9 consistent results. Retina stability for at least 24 hours. The above operation should be carried out in the dark, the test solution should be stored in the dark. High Performance Liquid Chromatography using Zorbax ODS 5 Column, acetonitrile as the mobile phase retention time of 5.47 points. The number of effective plates is 1L628 and the detection limit is 0.1a6.The linear range of the standard curve is 2. The relative standard deviation of the five determinations of the sample is 8.82%