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根据WHO1997年《口腔健康调查基本方法》中龋病诊断标准,分别选取dmft≥4(高龋组)和dmft=0(无龋组)的5～6岁儿童各30人,于上午9:00-11:00取所有儿童的非刺激性唾液,分别接种于变异链球菌、双歧杆菌、乳酸杆菌的选择性培养基,在37℃厌氧条件下培养48h后,取各选择培养基HE染色后倒置显微镜观察进行形态学鉴定。进而对各组各菌种菌落单位进行统计学分析。结果:5～6岁儿童口腔中均含有大量变异链球菌,明显高于其他两菌种(P<0.05),但高龋病组与无龋组之间无显著性差异(P>0.05);乳酸杆菌、双歧杆菌均以无龋组最高,与高龋组相比有显著性差异(P<0.05)。结论:乳酸杆菌、双歧杆菌是无龋儿童口腔中的优势菌,可能对降低该年龄段儿童的龋易感性有作用。
According to the WHO diagnostic criteria for oral health in 1997, 30 children aged 5 to 6 years with dmft≥4 (high caries group) and dmft = 0 (caries-free group), respectively, were selected at 9:00 am -11: 00 Take all children’s non-irritating saliva were inoculated on Streptococcus mutans, Bifidobacterium, Lactobacillus selective medium, cultured at 37 ℃ anaerobic conditions for 48h, take each selection medium HE staining After inverted microscope observation Morphological identification. Then each group of colony units for statistical analysis. Results: A large amount of Streptococcus mutans was found in the oral cavity of children aged 5 ~ 6 years old, which was significantly higher than those of the other two strains (P <0.05). However, there was no significant difference between caries control group and caries - free group (P> 0.05). Lactobacillus and Bifidobacterium were the highest in caries-free group, which were significantly different from those in high caries group (P <0.05). Conclusion: Lactobacillus and Bifidobacterium are the predominant bacteria in oral cavity of caries-free children, which may play an important role in reducing caries susceptibility in this age group.