论文部分内容阅读
[目的]研究HDS基因间的同源性及其进化关系。[方法]以毛果杨的HDS基因为研究对象,利用生物信息学软件及网站对其进行碱基分布、氨基酸组成、亲疏水性、保守区以及二级结构和三级结构的预测与分析。并与其他10个物种的HDS基因序列进行多重比对与进化分析。[结果]毛果杨HDS基因的单链mRNA序列长1956bp,编码由656个氨基酸组成的蛋白质,该蛋白质相对分子质量为72937.03,其中含量最高的是Leu,为10.50%;整个多肽中疏水性氨基酸只占39.63%,有10个亲水区和6个疏水区;与其他10个物种同源性多重比对发现同源性最高达99%。[结论]毛果杨HDS基因处于稳定状态,编码的蛋白为亲水性蛋白,在进化过程中是保守的;试验中获得的保守区段序列信息为新基因的克隆奠定了基础。
[Objective] The research aimed to study the homology and evolutionary relationship of HDS genes. [Method] With the HDS gene of Populus trichocalycium as the research object, bioinformatics software and its website were used to predict and analyze the base distribution, amino acid composition, hydrophilicity and hydrophobicity, conserved region, secondary structure and tertiary structure. Multiple alignment and evolutionary analysis were carried out with the HDS gene sequences of 10 other species. [Result] The single-stranded mRNA sequence of Pinus tabulaeformis HDS gene was 1956bp in length and encoded a protein consisting of 656 amino acids with a relative molecular mass of 72937.03. The highest content of Leu was 10.50%. The hydrophobic amino acid Accounting for only 39.63%. There are 10 hydrophilic regions and 6 hydrophobic regions. The homology with other 10 species shows homology up to 99%. [Conclusion] The results showed that the HDS gene was stable and the protein was hydrophilic, which was conserved during the evolutionary process. The sequence information of the conserved region obtained in the experiment laid the foundation for the cloning of the new gene.