Non-aqueous extracts of Curcuma mangga rhizomes induced cell death in human colorectal adenocarcinom

• 作 者: Gin Wah Hong Sok Lai Hong Guan Serm Lee Hashim Yaacob Sri Nurestri Abd Malek
• 机 构: Institute of Biological Sciences,Faculty of Science,University of Malaya,Meritus University,
• 出 处: Asian Pacific Journal of Tropical Medicine
• 发表日期: 2016年01期
• 关键词: Curcuma mangga Anticancer Apoptosis Cell cycle arrest HT29

Objective:To investigate the cytotoxic activity of the hexane and ethyl acetate extracts of Curcuma mangga rhizomes against human colorectal adenocarcinoma cell lines(HT29).Methods:The cytotoxic activity of the hexane and ethyl acetate extracts of Curcuma mangga rhizomes against human colorectal adenocarcinoma cell lines(HT29) was determined by using the SRB assay.Results:The ethyl acetate extract showed a higher cytotoxic effect compared to the hexane extract.Morphological changes of the HT29 cells such as cell shrinkage,membrane blebbling and formation of apoptotic bodies while changes in nuclear morphology like chromatin condensation and nuclear fragmentation were observed.Further evidence of apoptosis in HT29 cells was further supported by the externalization of phosphatidylserine which indicate early sign of apoptosis.Conclusions:The early sign of apoptosis is consistent with the cell cycle arrest at the G_0/G_1 checkpoint which suggests that the changes on the cell cycle lead to the induction of apoptosis in HT29.

Objective: To investigate the cytotoxic activity of the hexane and ethyl acetate extracts of Curcuma mangga rhizomes against human colorectal adenocarcinoma cell lines (HT29). Methods: The cytotoxic activity of the hexane and ethyl acetate extracts of Curcuma mangga rhizomes against human colorectal adenocarcinoma cell lines (HT29) was determined by using the SRB assay. Results: The ethyl acetate extract showed a higher cytotoxic effect compared to the hexane extract. Morphological changes of the HT29 cells such as cell shrinkage, membrane blebbling and formation of apoptotic bodies while changes in nuclear morphology like chromatin condensation and nuclear fragmentation were observed. Focus of apoptosis in HT29 cells was further supported by the externalization of phosphatidylserine which indicate early sign of apoptosis. Conclusions: The early sign of apoptosis is consistent with the cell cycle arrest at the G_0 / G_1 checkpoint which suggests that the changes on the cell cycle lead to the induction of apoptosis in HT29.