大鼠破骨细胞体外分离培养和鉴定

• 作 者: 张炜真 于世凤 郑麟蕃
• 机 构: 北京医科大学口腔医学院病理学教研室,北京医科大学口腔医学院病理学教研室,北京医科大学口腔医学院病理学教研室 北京100081,北京100081,北京100081
• 出 处: 解剖学报
• 发表日期: 1995年03期
• 关键词: 破骨细胞 体外分离培养 大鼠 骨吸收 醋酸酯酶 酸性磷酸酶染色 细胞悬液 降钙素 盖玻片 机械分离方法

采用机械分离方法,从新生大鼠长管骨分离破骨细胞获得成功。分离的破骨细胞具有如下特性:1.在体外迅速贴附于盖玻片或骨片;2.多核(一般为3～10个细胞核);3.伪足运动活跃,降钙素或降钙素基因相关肽可以抑制其伪足运动;4.酸性磷酸酶染色强阳性,酸性醋酸酯酶弱阳性,PAS中等阳性,5.细胞悬液与骨磨片共同培养,可以形成骨吸收陷窝。由此可见,本研究分离的细胞,符合破骨细胞的公认特性,故本法分离培养破骨细胞是可行的。

Using mechanical separation method, osteoclasts were isolated from the long bone of neonatal rat. Osteoclasts isolated have the following characteristics: 1. Rapidly attached to coverslips or bone fragments in vitro; 2. Multi-nuclei (typically 3 to 10 nuclei); 3. Artiodactyla active, calcitonin or calcitonin Superoxide dismutase can inhibit its pseudo-foot movement; 4. Acid phosphatase staining strong positive acid acetate weak positive, PAS moderately positive. 5 cell suspension and bone grinding co-culture can form bone resorption lacuna. It can be seen that the cells isolated in this study are in accordance with the recognized characteristics of osteoclasts. Therefore, it is feasible to separate and culture osteoclasts by this method.