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各种哺乳动物,包括灵长类,在妊娠期间受诱变剂作用,其子代的肿瘤发生率增高。动物出生前生殖细胞的遗传损伤,可使先天性畸形增多。因此评价环境中化学物质的危害时,必须注意对胚胎造成的损伤。胎肝血微核试验就是筛选、评价环境中诱变剂、致癌剂对胚胎造成损伤的一种方法。实验设计可用亚急性的最大无作用剂量或急性LD_(50)的1/5～1/10作为最高剂量。设三个剂量组、一个对照组,每组五只动物。妊娠第13天一次投与受试物,18小时后处死
Various mammals, including primates, are mutagenic agents during pregnancy and their offspring have an increased incidence of tumors. The genetic damage of germ cells before the birth of an animal can increase congenital malformations. Therefore, the assessment of environmental hazards of chemicals, we must pay attention to the damage caused by the embryo. Fetal Liver Blood Micronucleus Test is a method of screening and evaluating mutagens and carcinogens in the environment to damage embryos. Experimental design can be sub-acute maximum no dose or acute LD_ (50) 1/5 ~ 1/10 as the highest dose. Three dose groups, one control group, five animals per group. The test substance is administered once on the 13th day of gestation and sacrificed after 18 hours