论文部分内容阅读
To observe the effects of the micronutrients on oxidative and autoimmune destruction of islets so as to prevent a person from the onset and development of type 1 Diabetes Mellitus(T1DM), the interleukin-4 and interleukin-10 expressions of lymphocytes in peripheral blood and spleen of T1 DM rats were determined by flow cytometry. GSH-Px activity and MDA level in the rats' pancreas were measured using biochemical methods. The insulin contents in serum and β cell insulin secret storage were tested by RIA and IHC, respectively. There was an increase in the percentages of 1L-4 and IL-10 positive lymphocytes in the peripheral blood and spleen of the groups of rats supplemented with various combinations of micronutrients(p ＜0. 01 and p ＜0. 05, respectively); the blood glucose concentration decreased (p＜0.05); both the functional β cell in islets and the insulin content in pancreatic tissue increased(p＜0.05 and p＜0.01); the GSH-Px activity and MDA level of pancreas in the rats enhanced and decreased respectively(p＜0.01 and p＜0.05). The results suggest that micronutrients may alleviate the islet lesions by upregulating the expressions of IL-4 and IL-10 and lowering oxidative stress in diabetic rats.