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为了探讨肝切除后肝细胞生长因子 (HGF)及增殖细胞核抗原 (PCNA)在肝再生中的作用 ,制备大鼠肝切除后肝再生模型 ,应用免疫印迹法及免疫组织化学染色分别检测 HGF的分子形式及 PCNA的表达。结果发现 :HGF在肝切除后残存的肝组织中的含量在 12 h显著增加 ,是正常的 14倍。增加的水平维持到 2 4h。但 HGF的重链带低于检测水平。PCNA在肝切除后残存的肝组织中的表达明显升高 ,于术后 2 4h达高峰。阳性细胞高达 5 4%。提示 HGF在肝再生中的作用是微不足道的 ,而 PCNA可以作为一种肝细胞增生的指标。
To investigate the role of hepatocyte growth factor (HGF) and proliferating cell nuclear antigen (PCNA) in hepatic regeneration after hepatectomy, liver regeneration model after hepatectomy in rats was prepared. Immunohistochemistry and immunohistochemical staining were used to detect the molecules of HGF Form and PCNA expression. The results showed that the content of HGF in liver tissue remained after hepatectomy increased significantly at 12 h, which was 14 times of normal. The level of increase is maintained to 24 h. However, heavy chain of HGF is below detection level. The expression of PCNA was significantly increased in the remaining liver tissue after hepatectomy, reaching its peak at 24 hours after operation. Positive cells up to 54%. Suggesting that the role of HGF in liver regeneration is negligible, and PCNA can be used as an indicator of hepatocyte proliferation.