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The water-soluble polysaccharides isolated from the leaves of Panax ginseng C.A.Meyer were totally fractionated into one neutral fraction(GLPN-1),six acidic fractions(GLPN-2,GLPA-1a,GLPA-1b,GLPA-1c,GLPA-2 and GLPA-3) by a combination of ethanol precipitation,ion-exchange and gel permeation chromatography.All the fractions were analyzed by determining monosaccharide composition,molecular weight distribution.The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan.GLPA-1a,GLPA-1b and GLPA-2 mainly contained Gal,Ara,Rha and GalA.The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%.The molar ratios of Rha/GalA in GLPA-1a,GLPA-1b and GLPA-2 were 1:1,1:2 and 1:2.7,respectively,indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin.GLPA-1c was mainly composed of GalA(78%),belonging to homogalacturonan(HG)-rich pectin.GLPA-3 might be a glycoprotein.
The water-soluble polysaccharides isolated from the leaves of Panax ginseng CAMeyer were completely fractionated into one neutral fraction (GLPN-1), six acidic fractions (GLPN-2, GLPA-1a, GLPA- and GLPA-3) by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All fractions analyzed by determining monosaccharide composition, molecular weight distribution. Results show that GLPN-1 was mainly composed of Gal (38.1% and Ara (33.6%) and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha / GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1: 1,1: 2 and 1: 2.7, respectively, indicating that they belong to type- I rhamnogalacturonan (RG-I) -rich pectin.GLPA-1c was mainly composed of GalA (78%), belonging to homogalacturonan (HG) -rich pectin. GLPA-3 might be a glycoprotein.