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目的:研究三七总皂苷(PNS)对快速老化模型小鼠(SAMP8)学习记忆能力及脑内淀粉样前体蛋白(APP)基因转录水平的影响。方法:将SAMP8随机分为PNS高剂量组、PNS低剂量组、石杉碱甲(阳性对照组)组、模型组,各给药组分别按设定剂量灌胃给药,模型组给予等体积双蒸水灌服,连续8 w后应用Morris水迷宫、实时逆转录聚合酶链式反应(RT-PCR)的方法,检测PNS高、低剂量组SAMP8学习记忆和脑内APP mRNA含量的影响。结果:PNS能改善SAMP8的学习记忆能力(P<0.05或P<0.01),PNS高剂量还能显著降低其脑内APP mRNA含量(P<0.05)。结论:PNS能显著改善SAMP8的学习记忆能力,其作用途径可能与在转录水平下调APP基因的表达有关。
Objective: To study the effects of Panax Notoginseng Saponins (PNS) on the learning and memory ability and the gene transcription level of amyloid precursor protein (APP) in the mouse model of rapid aging (SAMP8). Methods: SAMP8 was randomly divided into high-dose PNS group, low-dose PNS group, huperzine A (positive control group), model group and each administration group. The mice were fed with distilled water twice a day for 8 weeks. Morris water maze and real-time reverse transcriptase polymerase chain reaction (RT-PCR) method were used to detect the effect of SAMP8 learning and memory and brain APP mRNA content in PNS high and low dose groups. Results: PNS could improve the learning and memory ability of SAMP8 (P <0.05 or P <0.01), and the high dose of PNS could significantly reduce the content of APP mRNA in brain (P <0.05). Conclusion: PNS can significantly improve the learning and memory ability of SAMP8, which may be related to the down-regulation of APP gene expression at transcriptional level.