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目的 研究慢性支气管炎 (慢支炎 )患者诱导痰中、肺泡巨噬细胞 (AM)培养上清液中及在氨茶碱和脂多糖 (LPS)干预下培养上清液中内皮素 (ET)的浓度变化 ,探讨AM源性ET在慢支炎及慢性阻塞性肺疾病 (COPD)病理进展过程中的作用。方法 选择慢支炎患者 14例 ,COPD患者 13例 ,同时选择 14名健康人作为正常对照。支气管肺泡灌洗技术收集支气管肺泡灌洗液 (BALF) ,对其细胞成分进行计数和分类 ;对其中 2 6例用高渗盐水诱痰法取痰标本 ,放射免疫法测定诱导痰中和AM培养上清液中的ET浓度。结果 (1)慢支炎组、COPD组BALF中细胞总数、中性粒细胞数、肺泡巨噬细胞数均明显高于正常对照组 (P均 <0 0 1) ;(2 )慢支炎组和COPD组AM培养上清液中ET浓度和诱导痰中ET浓度明显高于正常对照组 (P均 <0 0 1) ,但慢支炎组与COPD组之间差异无显著性 (P均 >0 0 5 ) ;(3)三组的诱导痰ET浓度与AM培养上清液ET浓度呈正相关 (r =0 741,P <0 0 1) ,与AM数呈正相关 (r =0 5 97,P <0 0 1) ;(4 )COPD组AM培养上清液ET浓度、诱导痰ET浓度均与一秒钟用力呼气容积占预计值 % (FEV1占预计值 % )呈负相关 (r =- 0 82 8,P <0 0 1;r =- 0 748,P <0 0 5 ) ;(5 )氨茶碱对AM培养上清液中ET浓度无影响 (P >0 0 5 ) ,而LPS使其浓度明显?
Objective To investigate the effect of endothelin (ET) in the culture supernatant induced by sputum, alveolar macrophage (AM) supernatant and aminophylline and lipopolysaccharide (LPS) in patients with chronic bronchitis (chronic bronchitis) , To explore the role of AM-derived ET in the pathogenesis of chronic myoepithelial and chronic obstructive pulmonary disease (COPD). Methods Forty patients with chronic myositis, 13 patients with COPD and 14 healthy controls were selected as normal controls. Bronchoalveolar lavage technique was used to collect bronchoalveolar lavage fluid (BALF) and to count and classify the cellular components. Twenty-six of the patients were treated with hypertonic saline induced phlegm method and sputum and AM were induced by radioimmunoassay ET concentration in supernatant. Results (1) The numbers of total cells, neutrophils and alveolar macrophages in BALF of COPD group and COPD group were significantly higher than those of the normal control group (all P <0.01). (2) (P <0.01). However, there was no significant difference between chronic bronchitis group and COPD group (P> (3) There was a positive correlation between induced sputum ET concentration and ET concentration in AM supernatant (r = 0 741, P 0 01), and positive correlation with AM number (r 0 057, P <0.01). (4) ET concentration and induced sputum ET concentration in AM culture supernatant of COPD group were negatively correlated with forced expiratory volume in one second (FEV1% predicted) (r = - 0 82 8, P <0.01; r = - 0 748, P <0 05); (5) There was no effect of aminophylline on ET concentration in AM supernatant (P> 0.05) LPS make its concentration significantly?