论文部分内容阅读
以黑农44(高脂肪品种)大豆品种作为试验材料,以不同方式在连作大豆根际土壤中接种摩西管柄囊霉(Funneliformis mosseae)和大豆根腐病原菌尖孢镰刀菌(Fusarium oxysporum)。采用传统形态学方法检测Fu.mosseae和F.oxysporum侵染大豆植株根系情况。利用实时荧光定量PCR技术分析Fu.mosseae和F.oxysporum DNA含量变化。结果表明:Fu.mosseae和F.oxysporum均能侵染大豆植株根系,但接种Fu.mosseae的大豆植株根系及根际土壤样品中F.oxysporum DNA含量显著降低,表明Fu.mosseae对F.oxysporum具有强烈的抑制作用。本研究为进一步研究AM真菌克服大豆连作障碍提供了理论依据。
Taking Heinong 44 (high-fat variety) soybean varieties as test materials, Funneliformis mosseae and soybean root rot pathogen Fusarium oxysporum were inoculated in different ways in soybean rhizosphere soil. Traditional morphological methods were used to detect the root system of soybean plants infected by Fu.mosseae and F. oxysporum. The changes of DNA content of Fu.mosseae and F. oxysporum were analyzed by real-time fluorescence quantitative PCR. The results showed that both Fu.mosseae and F. oxysporum could infect the roots of soybean plants, but the contents of F. oxysporum DNA in the roots and rhizosphere soil of soybean plants inoculated with Fu.mosseae were significantly decreased, indicating that Fu.mosseae had Strong inhibitory effect. This study provides a theoretical basis for further research on AM fungi to overcome the continuous cropping obstacles in soybean.