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目的 研究胃粘膜萎缩和广泛肠化的细胞增殖与凋亡的定量关系。 方法 CSG14例,CAG11例,CAG+IM20例,对照5例,分别作HE染色,ISEL检测细胞凋亡,免疫组化PCNA和bcl-2抗体染色。求各组增殖指数(PI)、凋亡指数(AI)、凋亡增殖比和凋亡强度。 结果 各组PI和AI分别是:15.4％,24.8％,56.9％和8.4％,5.4％,11.6％,1.9％和1.4％;在CSG,CAG凋亡和增殖呈正相关(r=0.5475和0.5839),CAG凋亡强度最大;CAG+IMPI最高,但凋亡和增殖呈负相关(r=-0.6742),凋亡强度异常低,不足对照组的1/5,CSG的1/10,CAG的1/4。 结论 CAG的萎缩可能是过度凋亡所致;CAG+IM则出现凋亡障碍,过度增殖,与异型增生和癌的细胞生物学表现相似。
Objective To study the quantitative relationship between cell proliferation and apoptosis in gastric mucosal atrophy and extensive intestinal metaplasia. Methods 14 cases of CSG, 11 cases of CAG, 20 cases of CAG + IM and 5 cases of control were stained with hematoxylin and eosin (HE), respectively. The expressions of PCNA and bcl-2 were detected by ISEL. Proliferation index (PI), apoptotic index (AI), apoptosis ratio and apoptosis intensity were calculated. Results The PI and AI in each group were 15.4%, 24.8%, 56.9% and 8.4%, 5.4%, 11.6%, 1.9% and 1.4% respectively. There was a positive correlation between apoptosis and proliferation of CSG and CAG (r = 0.5475 and 0.5839) CAG + IMPI was the highest, but there was a negative correlation between apoptosis and proliferation (r = -0.6742). The apoptosis intensity was abnormally low, which was less than 1/5 in the control group, 1/10 in CSG, 1 / 4. Conclusions The atrophy of CAG may be caused by excessive apoptosis. CAG + IM may display apoptosis disorder and hyperproliferation, which is similar to the cell biology of dysplasia and carcinoma.