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蚀斑实验是研究病毒感染动力学最常用的方法,也是研究病毒与细胞相互作用的方法之一.但是,病毒颗粒在溶液中的随机分布导致蚀斑在细胞层上的形成位置难以确定,因此难以对蚀斑进行长时间的示踪观察.本文通过设计微流控芯片内微通道的结构和尺寸,实现了对样品的定点微注射,研究了喷嘴尺寸和流体流速对微注射的影响.将该芯片与开放的细胞培养区域结合,成功实现了牛痘病毒的定点微注射;对牛痘病毒侵染宿主细胞形成的蚀斑及蚀斑的变化进行长时间示踪,研究了牛痘病毒在宿主细胞之间的传播速率.本文中设计的微注射芯片解决了长期示踪蚀斑的难题,为病毒感染动力学研究及病毒与细胞相互作用研究提供了一个新的方法和平台.
Plaque testing is one of the most commonly used methods for studying the kinetics of virus infection and is one of the means of studying the interaction of viruses with cells. However, the random distribution of virus particles in solution results in the difficulty of determining the location of plaque formation on the cell layer It is difficult to trace the plaque for a long time.In this paper, by micro-channel structure and size of the microfluidic chip designed to achieve the micro-injection of the sample sentinels, the nozzle size and fluid flow velocity on the microinjection of The chip with open cell culture area, the successful realization of the vaccinia virus microinjection of spots; Vaccinia virus infection in host cells formed by plaque and plaque changes were traced for a long time to study the vaccinia virus in host cells Between the propagation velocity of the micro-injection chip designed in this article to solve the problem of long-term tracing plaque, viral infection kinetics and virus-cell interaction studies provide a new method and platform.