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利用离心、盐析及ＤＥＡＥ－纤维素、羟基磷灰石、葡聚糖－Ｇ１００多步层析法将牛睾丸ＡＲ分离纯化，醛糖还原酶（ａｌｄｏｓｅｒｅｄｕｃｔａｓｅ，ＡＲ），并利用体外酶动力学方法测定其底物特异性，为寻找有效的醛糖还原酶抑制剂提供线索。结果：纯化的酶经ＳＤＳ－ｐＡＧＥ电泳鉴定示一单一蛋白条带，牛睾丸ＡＲＭｒ约为（４００００±１０００）ｕ，ＡＲ底物特异性，按１／ｋｍ大小依次排列为：Ｐ－硝基苯醛，ＤＬ－甘油醛，Ｄ－葡萄糖醛酸，Ｄ－木糖，Ｄ－半乳糖，Ｄ－葡萄糖。
The bovine testicular AR was isolated and purified by centrifugation, salting-out and DEAE-cellulose, hydroxyapatite and dextran-G100 multi-step chromatography. The aldosereductase (AR) Determination of its substrate specificity, in order to find effective aldose reductase inhibitors provide clues. Results: The purified enzyme showed a single protein band by SDS-PAGE electrophoresis. The average value of ARMr in bovine testis was (40 000 ± 1000) u, the substrate specificity of AR was arranged in order of 1 / km as follows: P-nitrobenzene Aldehyde, DL-glyceraldehyde, D-glucuronic acid, D-xylose, D-galactose, D-glucose.