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目的：研究肿瘤坏死因子－α（ＴＮＦ－α）在肠源性内毒素血症中的作用及丹参防治肝损伤机制。方法：用硫代乙酰胺（ＴＡＡ）诱发大鼠急性肝损伤，检测血浆内毒素、ＴＮＦ－α及丙氨酸氨基转移酶（ＡＬＴ）水平变化，并与丹参治疗组进行比较。结果：ＴＡＡ诱发急性肝损伤大鼠血浆内毒素及ＴＮＦ－α含量升高，二者呈正相关；血浆ＡＬＴ亦升高，与ＴＮＦ－α亦呈正相关。丹参治疗组大鼠血浆ＴＮＦ－α及ＡＬＴ水平均低于肝损伤对照组。结论：ＴＡＡ引起肠源性内毒素血症，内毒素促进ＴＮＦ－α释放，ＴＮＦ－α进一步介导肝损伤。丹参防治肝损伤作用机制之一可能是通过降低ＴＮＦ－α水平而实现的
Objective: To study the role of tumor necrosis factor-α (TNF-α) in intestinal endotoxemia and the mechanism of Salvia miltiorrhiza against liver injury. METHODS: Acute hepatic injury was induced in rats by thioacetamide (TAA). Changes of plasma endotoxin, TNF-α, and alanine aminotransferase (ALT) levels were measured and compared with those of the Salvia miltiorrhiza treatment group. RESULTS: The plasma levels of endotoxin and TNF-α in rats with acute liver injury induced by TAA were positively correlated with each other. Plasma ALT was also increased, which was also positively correlated with TNF-α. The plasma levels of TNF-α and ALT in the salvia miltiorrhiza treatment group were lower than those in the liver injury control group. Conclusion: TAA causes intestinal endotoxemia, endotoxin promotes TNF-α release, and TNF-α further mediates liver injury. One of the mechanisms of Salvia miltiorrhiza for preventing and treating liver injury may be achieved by reducing the level of TNF-α