论文部分内容阅读
目的探讨用Walker256乳腺癌细胞建立大鼠胫骨癌痛模型的可行性。方法 45只SD雄性大鼠随机均分为肿瘤组、假手术组和正常对照组。将Walker256大鼠乳腺癌细胞事先接种于幼鼠腹腔;7d后收集腹水瘤细胞(5×103个/μl)并经髁间隆起注入肿瘤组大鼠胫骨上段,制备骨癌痛模型。观察建模后22d内的疼痛行为学和22d时胫骨CT影像学变化。结果肿瘤组造模后第6天开始,机械性痛阈值明显低于假手术组和正常对照组(P<0.05)。三组热痛觉过敏潜伏期差异无统计学意义(P>0.05)。结论用Walker 256大鼠乳腺癌细胞成功建立了大鼠胫骨癌痛模型。
Objective To explore the feasibility of using Walker256 breast cancer cells to establish rat model of tibial cancer pain. Methods 45 SD male rats were randomly divided into tumor group, sham operation group and normal control group. Walker256 rat breast cancer cells were inoculated into the peritoneal cavity of mice prematurely. After 7 days, ascites tumor cells (5 × 103 / μl) were collected and injected into the upper part of the tibia of rats in the tumor group via intercondylar eminence. Pain behavior within 22 days after modeling and changes of tibial CT imaging at 22 days were observed. Results On the 6th day after modeling, the threshold of mechanical pain was significantly lower in the tumor group than in the sham operation group and the normal control group (P <0.05). There was no significant difference in latency of thermal hyperalgesia between the three groups (P> 0.05). Conclusion The rat model of tibial cancer pain was successfully established in Walker 256 rat breast cancer cells.