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采用带菌牙签活体茎秆穿刺接种法和离体叶片菌饼接种法,以3个品种的油菜为供试寄主,对陕西省5个地区10个县的油菜和其它5种寄主共495个核盘菌分离株进行了致病性测定。结果显示,两种接种方法接种3个不同油菜品种所反映的不同分离株的致病趋势一致。离体叶片菌饼接种蓉油12号除来自勉县和城固县的2个分离株不能致病外,其余493个分离株均能致病,但各分离株所致病斑直径差异很大,为0.5～42.6mm,其中病斑直径在10～40mm的分离株占94.7%,属于优势群体。同一地区甚至同一地块均存在不同致病力的分离株,不同寄主来源的分离株在油菜离体叶片上均能致病。说明核盘菌种内存在明显的致病性分化,这种分化与地理来源和寄主来源均无明显的相关性。
In this study, we used three methods of inoculation of stalk of stalk with live toothpicks and inoculation method of in vitro leafbacterium cake. Three varieties of rape were used as hosts for testing, and a total of 495 nuclear discs of rapeseed and five other hosts in 10 counties of Shaanxi Province Isolates were tested for pathogenicity. The results showed that the two isolates inoculated with three different varieties of rape reflected the same trend of different isolates. The results showed that 493 isolates could be induced by pathogen inoculation in Rongyou 12 except for two isolates from Mianxian and Chenggu county, but the pathogenic diameters of isolates were very different , Which ranged from 0.5 to 42.6 mm. 94.7% isolates with lesion diameters of 10-40 mm belonged to the dominant group. In the same area and even in the same plots, there were isolates with different pathogenicity. The isolates from different host plants could cause disease in rape isolated leaves. The results showed that there was obvious pathogenic differentiation in S. sclerotiorum, which had no obvious correlation with geographical origin and host origin.