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目的探索人结肠癌细胞SW480转染4IgB7-H3后对糖基转移酶β3GnT8和细胞表面多聚乳糖胺糖链表达的影响。方法脂质体介导的重组表达载体pIRES2-EGFP/4IgB7-H3转染SW480细胞，筛选并获得稳定表达4IgB7-H3的亚克隆细胞株；RT-PCR、Western Blot检测β3GnT8的表达；流式细胞术检测多聚乳糖胺寡糖链的表达。结果成功获得稳定表达4IgB7-H3的亚克隆细胞株SW480/4IgB7-H3；转染4IgB7-H3的SW480细胞中β3GnT8和多聚乳糖胺糖链的表达均明显下调（P＜0.05）。结论人肠癌细胞中4IgB7-H3过表达能抑制β3GnT8的表达，进而抑制多聚乳糖胺糖链的表达。“,”Objective To explore the expressions of glycosyltransferaseβ3GnT8 and polylactosamine on cell surface in human colon cancer cell line SW480 transfected with 4IgB7-H3.Methods Liposome-mediated method transfected recombinant expression carrier pIRES2-EGFP/4IgB7-H3 into SW480 cells;RT-PCR,Western Blot detected the expression of 4IgB7-H3 andβ3GnT8 at the mRNA and protein levels;Flow cytometry detcted lactosamine oligosaccharide chain expression.Results 4IgB7-H3 recombinant expression vector was successfully constructed.Then stably transfected SW480 cell line was got.Both the expressions ofβ3GnT8 and poly lactose amine sugar chain decreased signiifcantly(P<0.05) after transfection.Conclusion 4IgB7-H3 overexpression in human colorectal cancer cells can inhibitβ3GnT8 production,and thereby inhibit poly galactosamine extension.