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目的了解乙醇对雌雄SD大鼠急性肝损伤程度差异以及赶黄草对雌性SD大鼠急性乙醇肝损伤的辅助保护作用。方法受试物1、2组雌性SD大鼠分别一次灌胃赶黄草和纯牛奶,0.5 h后除阴性对照组外,受试物1、2组和雌雄SD大鼠模型组分别一次性灌胃50%乙醇,禁食16 h,麻醉取血清检测谷丙转氨酶(ALT)和谷草转氨酶(AST)的含量,并对肝脏作组织病理学检查。结果病理显示,模型组雌性SD大鼠酒精性肝损伤程度明显比雄性严重(P<0.01),饮酒前饮用赶黄草或牛奶对肝脏都有显著保护作用(P<0.05、P<0.05)。结论在酒精性肝损伤动物模型中,对动物性别的限制是十分必要的。在未长期服用赶黄草的情况下,饮酒前饮用赶黄草与饮用牛奶相比,两者对肝脏保护效果无明显差异。
OBJECTIVE To investigate the difference of ethanol-induced acute liver injury in female and male SD rats and the auxiliary protective effect of Phellinus igniarius on acute alcoholic liver injury in female SD rats. Methods 1, 2 groups of female Sprague-Dawley rats were fed ganoderma lucidum and pure milk, 0.5 h after the addition of the negative control group, the test group 1, 2 groups and male and female SD rat model group were one-time irrigation Stomach 50% ethanol, fasting for 16 h, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined by anesthesia, and liver histopathological examination. Results Pathological examination showed that the degree of alcoholic liver injury in female SD rats was significantly higher than that in male rats (P <0.01). The consumption of Phellinus igniarius or milk before drinking had a significant protective effect on the liver (P <0.05, P <0.05). Conclusion In the animal model of alcoholic liver injury, animal sex restriction is very necessary. In the absence of long-term use of Phellincu leucois, the case before drinking drink Phellodendron grass compared with drinking milk, the two have no significant difference in the protective effect on the liver.