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Acorus tatarinowii Schott is a traditional Chinese medicine plant and has multiple bioactivities in medicine and pesticide field. In this study, the antifungal compound l,2-dimethoxy-4(2-propenyl) benzene was isolated from A. tatarinowii Schott by activity-directed isolation method, and the inhibitory activity of the extract and l,2-dimethoxy-4(2-propenyl) benzene against seven plant pathogenic fungi was evaluated. The results showed that the extract and l,2-dimethoxy-4(2-propenyl) benzene had high inhibitory activity against hyphal growth of Thielaviopsis paradoxa (de Seynes) V. Hohnel, Pestalotia mangiferae P. Henn., Fusarium oxysporum f. sp. niveum (E. F. Smith) Syn. et Hans., Alternaria alternate Tanaka, Colletotrichum musae (Berk et Curt) V. Arx, Sphaceloma fawcettii Jenk., and Mycosphaerella sentina (Fr.) Schroter. The EC50 values of extract were 1.6162, 1.6811, 1.1253,3.5771,1.7024, 2.2284, and 2.2221 g L-1, respectively, and the EC50 values of l,2-dimethoxy-4(2-propenyl) benzene were 0.1021,0.0997,0.0805,0.1742,0.1503,0.1853, and 0.1924 g L-1, respectively. l,2-Dimethoxy-4(2-propenyl) benzene also inhibited spores germination of T. paradoxa (de Seynes) V. Hohnel and F. oxysporum f. sp. niveum (E. F. Smith) Syn. et Hans., with the inhibitory rates of 98.81 and 100% at a concentration of 0.4 g L(-1) after 8 h, respectively. l,2-Dimethoxy-4(2-propenyl) benzene is a potential botanical antifungal agent for controling of plant fungal diseases.