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研究了4种不同来源脂肪酶中壳聚糖降解活力最高的脂肪酶降解壳聚糖的特性。结果表明该酶对不同脱乙酰度(DD)值壳聚糖均有水解作用,其中对CHS3的水解活力最高,最适作用温度均为60℃,但最适pH不同。脂肪酶水解不同DD值壳聚糖的反应均遵循Michaelis-Menten方程,中等DD值壳聚糖与脂肪酶的结合能力较强。红外光谱分析表明,脂肪酶在催化壳聚糖水解过程中,壳聚糖的侧链结构未发生改变。
The degradation of chitosan by lipase with the highest degradation activity of chitosan from four different lipases was studied. The results showed that the enzyme hydrolyzed chitosan with different degree of deacetylation (DD). Among them, the hydrolytic activity of CHS3 was the highest, and the optimum temperature was 60 ℃, but the optimum pH was different. Lipase hydrolysis of different DD values of chitosan reaction follow the Michaelis-Menten equation, moderate DD chitosan and lipase binding ability. Infrared spectroscopy analysis showed that the side chain structure of chitosan did not change when lipase catalyzed the hydrolysis of chitosan.