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目的:建立HPLC同时测定通宣理肺胶囊中柚皮苷、橙皮苷、黄芩苷的含量。方法:色谱柱Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸溶液(21∶79),检测波长280 nm,流速1.0 mL.min-1,柱温30℃。结果:柚皮苷进样量在0.156～1.408μg线性关系良好(r=0.999 8,n=5),平均回收率99.56%,RSD 0.88%(n=9);橙皮苷进样量在0.145～1.301μg线性关系良好(r=0.999 7,n=5),平均回收率99.83%,RSD 0.66%(n=9)。黄芩苷进样量在0.359～3.233μg线性关系良好(r=0.999 9,n=5),平均回收率为98.94%,RSD 1.55%(n=9);结论:该方法操作简便,准确可靠,可作为通宣理肺胶囊的质量控制。
Objective: To establish an HPLC method for simultaneous determination of naringin, hesperidin and baicalin in Tongxuanlifei Capsules. Methods: Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-0.1% phosphoric acid solution (21:79) . Results: The linear range of naringin injection was 0.156 ~ 1.408μg (r = 0.999 8, n = 5), the average recovery was 99.56% and the RSD was 0.88% (n = 9) ~ 1.301μg linear relationship (r = 0.999 7, n = 5), the average recovery was 99.83%, RSD 0.66% (n = 9). The average recovery of baicalin was 0.359-3.233μg (r = 0.999 9, n = 5), the average recovery was 98.94% and the RSD was 1.55% (n = 9) .Conclusion: The method is simple, accurate and reliable, It can be used as the quality control of Xuanfei lung capsule.