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目的测定广西不同产地龙眼花药材中的总黄酮。方法以总黄酮含量为考查指标,采用正交试验优选提取工艺;以芦丁为对照品,采用紫外可见分光光度法(UV)测定,测定波长505nm。结果总黄酮的线性范围为9.904～59.424μg·mL-1(r=0.9995),平均加样回收率为101.0%,RSD=1.76%(n=6)。广西不同产地的龙眼花药材中,以大新的龙眼花总黄酮的含量最高,其余各地龙眼花总黄酮含量各有差异。结论所用方法快速、准确、灵敏,可用于龙眼花药材的质量控制。
Objective To determine the total flavonoids in the longan anthers of Guangxi. Methods The content of total flavonoids was taken as the test index, and the orthogonal experiment was adopted to optimize the extraction process. Rutin was used as the reference substance and the UV wavelength was 505nm by UV-visible spectrophotometry (UV). Results The linear range of total flavonoids was 9.904-59.424μg · mL-1 (r = 0.9995). The average recovery was 101.0% with RSD = 1.76% (n = 6). Longan anthers from different areas of Guangxi, Dah Sing with the highest content of total flavonoids, the rest of the total flavonoids content of longan flowers are different. Conclusion The method used is rapid, accurate and sensitive and can be used for the quality control of longan anther.