Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene

• 作 者: Jianting HU Hongzhao LI Taopin
• 机 构: Department of Urology, Tong]i Hospital, Tongji Medical College, Huazhong University of Science and T
• 出 处: 华中科技大学学报（医学）（英德文版）
• 发表日期: 2008年2期
• 关键词: bladder transitional cell carcinoma DNA methylation Western-blotting RASSF1A gon

To investigate the relationship between the expression of RASSFIA protein and promoter hypermethylation of RASSFIA gene, RASSFIA protein expression was measured by West blot- ting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carci- noma (BTCC). The promoter methylation in BTCC and normal bladder tissues was detected by me- thylation-specific PCR (MSP). The results showed that the expression level of RASSFIA protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not corre- lated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF1A protein. It is con- cluded that RASSFIA gene promoter methylation may contribute to the low level or loss of RASSFIA protein expression, the inactivation of RASSFIA gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.