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目的探讨整合型ＨＢＶ－ＤＮＡ前Ｃ区基因突变与肝细胞癌的关系。②方法应用聚合酶链反应－单链构象多态技术，对２９例肝癌、１２例慢性肝硬化和１４例非肝病病人的肝组织内整合型ＨＢＶ－ＤＮＡ前Ｃ区进行突变分析。③结果肝癌、肝硬化和正常肝组织中整合型ＨＢＶ－ＤＮＡ阳性率分别为９６．５５％，６６．６７％和１４．２９％，组间比较差异有显著性（χ２＝２９．４３，Ｐ＜０．００１），３组整合型ＨＢＶ－ＤＮＡ前Ｃ区突变率分别为４１．６％，２５．０％和０，组间比较差异有显著性（χ２＝１２．４４，Ｐ＜０．０５）。④结论肝组织中整合型ＨＢＶ－ＤＮＡ前Ｃ区突变与肝细胞癌的发生和发展相关。
Objective To investigate the relationship between HBV DNA pre-C gene mutation and hepatocellular carcinoma (HCC). Methods The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the mutation of the pre-HBV DNA integrated region in 29 liver cancer patients, 12 chronic liver cirrhosis patients and 14 non-liver disease patients. Results The positive rates of integrated HBV-DNA in hepatocellular carcinoma, cirrhosis and normal liver tissues were 96.55%, 66.67% and 14.29%, respectively, with significant difference between the two groups (χ2 = 29.43, P <0.001). The mutation rates of the pre-C region of integrated HBV-DNA in three groups were 41.6%, 25.0% and 0 respectively, with significant difference between the two groups (χ2 = 12.44, P <0. 05). ④ Conclusion The integration of HBV-DNA pre-C mutation in liver tissue is related to the occurrence and development of hepatocellular carcinoma.