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目的研究COX-2和凋亡相关基因Bcl-2、Bax在二十二碳六烯酸(DHA)抑制人胰腺癌细胞体外增殖中表达的变化,探讨DHA抑制肿瘤细胞的作用机制。方法采用四甲基偶氮唑蓝(MTT)实验、流式细胞技术对细胞增殖、细胞凋亡、细胞周期以及肿瘤相关蛋白COX-2和凋亡基因Bcl-2、Bax进行分析。结果DHA能以时间-剂量依赖关系抑制人胰腺癌细胞增殖,同时诱导细胞凋亡。DHA对正常人成纤维细胞的增殖无明显影响。流式细胞仪检测显示:G0-G1期细胞比例增高,S期细胞比例下降。DHA作用24h后,胰腺癌细胞的COX-2、Bcl-2、Bax蛋白表达下降。结论DHA下凋COX-2、Bcl-2、Bax的表达可能是其抑制胰腺癌细胞增殖的作用机制之一。
Objective To investigate the changes of COX-2 and Bcl-2 and Bax in inhibiting the proliferation of human pancreatic cancer cells induced by docosahexaenoic acid (DHA), and to explore the mechanism of DHA inhibiting tumor cells. Methods The proliferation, apoptosis, cell cycle, COX-2 and Bcl-2, Bax were analyzed by MTT assay and flow cytometry. Results DHA inhibited the proliferation of human pancreatic cancer cells in a time-and dose-dependent manner and induced apoptosis. DHA had no significant effect on the proliferation of normal human fibroblasts. Flow cytometry showed that: the proportion of G0-G1 phase cells increased, the proportion of S phase cells decreased. After DHA for 24 hours, the expression of COX-2, Bcl-2 and Bax in pancreatic cancer cells decreased. Conclusions The expression of COX-2, Bcl-2 and Bax in DHA may be one of its mechanisms of inhibiting the proliferation of pancreatic cancer cells.