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以实生欧李群体为试材,采用扫描电镜观测方法,研究同一生境条件下欧李花粉超微形态结构以及花粉和成熟期果实中矿质元素含量变化及其相关关系,以期为欧李矿质营养代谢机制和钙高效基因型早期筛选提供理论参考。结果表明:欧李花粉形状为长球形或超长球形,极面观为三裂圆形,具三孔沟,按NPC系统分类属于N3P4C5型;花粉外壁纹饰呈穿孔纹饰、条纹-穿孔纹饰和条纹状纹饰三种类型;不同株系在花粉形状、大小、表面纹饰、极轴长(P)、赤道轴长(E)、P/E以及花粉和果实中矿质元素含量等生理形态指标变异较大。相关分析结果表明,花粉中Ca、Mg元素与花粉极轴和赤道轴呈显著或极显著正相关,花粉中K元素与赤道轴和P/E分别呈极显著负相关和显著正相关,花粉中Fe元素与赤道轴呈显著正相关;果实中Ca、K元素与极轴呈显著正相关;花粉和果实中Mn元素与P/E均呈显著负相关。
In this study, the real-life Oryza sativa populations were used as experimental materials and the ultrastructure of Oryza sativa L. ultrastructure and pollen and mature fruits in the same habitat were studied by scanning electron microscopy. Mechanism and efficient screening of calcium genotypes provide a theoretical reference. The results showed that the pollen morphology of longan or long spheroids was polar and the surface of the pollen was triple-cleaved with a three-hole ditch, classified as N3P4C5 according to the NPC system. The pollen exine ornamentation was perforated ornamentation, striped-perforation ornamentation and streaks There were three major morphological variations of pollen morphology and pollen morphology in pollen grains. Pollen morphology, size, surface ornamentation, polar axis length (P), equatorial axis length (E), P / . Correlation analysis showed that there was a significant or very significant positive correlation between Ca and Mg in pollen and pollen and equatorial axes. K in pollen had a significantly negative correlation with significant positive correlation with equatorial axis and P / E. Pollen There was a significant positive correlation between Fe element and the equatorial axis. There was a significant positive correlation between element Ca and K in the fruit and polar axis. There was a significant negative correlation between element Mn and P / E in pollen and fruit.