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目的 :研究微电极放大器中的 5 0Hz滤波电路对心肌细胞动作电位波形及各项参数的影响。方法 :将心肌细胞动作电位经玻璃微电极、微电极放大器、微分器、A/D转换器输入微型计算机。在使用和不使用微电极放大器中5 0Hz滤波功能两种情况下 ,对心肌细胞动作电位波形进行比较分析 ,并用快速傅立叶变换进行频谱分析。结果 :使用微电极放大器中 5 0Hz滤波功能情况下 ,动作电位波形在 0期严重失真 ,上升时间延长 ,最大上升速度减小 ,其它参数无显著变化。结论 :心肌细胞动作电位波形中含有较大 5 0Hz信号成分 ,在研究心肌细胞动作电位信号时 ,不能使用 5 0Hz滤波器。如果使用 5 0Hz滤波器 ,会造成动作电位波形严重失真 ,影响实验结果
Objective: To study the effect of 50Hz filter circuit in microelectrode amplifier on the action potential waveforms and parameters of cardiomyocytes. Methods: The action potentials of cardiomyocytes were input to microcomputer by glass microelectrode, micro-electrode amplifier, differentiator and A / D converter. In both cases, the action potential waveforms of cardiomyocytes were compared with and without the 50Hz filtering function in the microelectrode amplifier, and the frequency spectrum was analyzed by Fast Fourier Transform. Results: Under the condition of 50Hz filter function in the microelectrode amplifier, the action potential waveform was severely distorted in phase 0, the rise time was prolonged, the maximum rise speed was reduced, and other parameters did not change significantly. CONCLUSION: The waveform of action potential of cardiomyocytes contains large 50Hz signal components. In the study of cardiomyocyte action potential signal, the 50Hz filter can not be used. If the use of 50Hz filter, will result in serious distortion of the action potential waveform, affecting the experimental results