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为了测定新生小鼠发育过程中脑组织中ＮＭＤＡ受体亚单位蛋白的表达水平，用ＮＭＤＡ受体亚单位特异的抗体，通过引入成年小鼠皮层组织系列稀释的样品作标准，建立了定量的免疫印迹分析法。并对出生后不同日龄（２～３５ｄ）新生小鼠前脑和小脑组织中ＮＭＤＡ受体ζ１、ε１和ε２亚单位蛋白进行了测定。结果表明，ζ１、ε１和ε２三个亚单位在大鼠前脑组织中呈相似的增加趋势，只是ε１的表达明显的滞后。而小脑此３种亚单位的表达绝对量均明显低于前脑组织，并且趋势各不相同：ζ１经明显的下降后维持在一定的水平，ε１呈增加趋势，ε２出生后５ｄ即有相当的表达，以后下降，３周后几乎不能测出。上述结果与相应的ｍＲＮＡ半定量分析结果基本一致，并且与大鼠同类研究结果大致吻合
In order to determine the level of NMDA receptor subunit protein expression in brain tissue of newborn mice, a quantitative immunization was established by introducing serially diluted samples of adult mouse cortical tissue with NMDA receptor subunit-specific antibodies Imprinting method. The ζ1, ε1 and ε2 subunit proteins of NMDA receptor in the forebrain and cerebellum of neonatal mice of different ages (2-35 d) were measured after birth. The results showed that ζ1, ε1 and ε2 three subunits in forebrain in rats showed a similar trend of increase, but the expression of ε1 lag significantly. However, the absolute amounts of these three subunits in the cerebellum were significantly lower than those in the forebrain, and the trends were different: ζ1 was maintained at a certain level after a significant decrease, ε1 showed an increasing trend, and ∈2 had a comparable After the decline, after 3 weeks can hardly be measured. The above results and the corresponding mRNA semiquantitative analysis of the results are basically the same, and roughly consistent with the results of similar studies in rats