论文部分内容阅读
目的评价蜘蛛香的黄酮类提取物和缬草三酯提取物的药效。方法采用热板法、醋酸扭体法研究提取物的镇痛作用;炭末法、家兔离体肠肌研究提取物对胃肠运动的影响;高架十字迷宫和明暗箱用于研究提取物抗焦虑实验。结果在给药60、120 min时黄酮低剂量对提高小鼠痛阈值具有一定作用(P<0.05),总黄酮中、高剂量均能显著提高小鼠痛阈值(P<0.01),缬草三酯各给药组对小鼠热刺激致痛的抑制作用不显著,总黄酮提取物高剂量能显著延长扭体反应的潜伏期和减少醋酸所致的小鼠扭体次数(P<0.01),缬草三酯提取物各剂量的作用不显著(P>0.05);黄酮提取物中、高剂量和缬草三酯提取物高剂量都能显著增加小肠炭末的推进率(P<0.01),黄酮提取物高剂量对家兔离体肠肌均有显著的舒张作用(P<0.01),缬草三酯高剂量对离体肠肌具有一定的舒张作用(P<0.05);蜘蛛香总缬草三酯提取物高剂量能显著增加小鼠进入开臂次数与进入各臂总次数的百分比(OE)、在开臂内的活动时间与进入各臂的总活动时间的百分比(OT)值(P<0.01),中、高剂量可明显增加小鼠穿箱次数和明箱活动时间(P<0.01),而总黄酮提取物各剂量对增加小鼠穿箱次数和明箱活动时间作用不显著。结论蜘蛛香的总黄酮类成分是镇痛、改善胃肠运动的主要成分,缬草三酯类成分是抗焦虑药效的主要成分。
Objective To evaluate the efficacy of spider incense flavonoids extract and valerian triester extract. Methods The hot plate method and acetic acid writhing method were used to study the analgesic effect of extract. The carbon powder method was used to study the effect of extract on gastrointestinal motility in isolated intestinal muscle of rabbits. Elevated plus maze and light and dark box were used to study the anti-anxiety experiment. Results Low dose of flavonoids could increase the pain threshold of mice at 60 and 120 min (P <0.05). The medium and high dose of total flavonoids could significantly increase the pain threshold of mice (P <0.01) Estrogen could inhibit the hyperthermia induced pain in mice. The high dosage of total flavonoids could prolong the latent period of writhing reaction and reduce the number of writhing induced by acetic acid (P <0.01) (P> 0.05). The medium and high dose of flavonoids extract and high dose of Valerian triester extract could significantly increase the propulsion rate of small intestine carbon (P <0.01), the content of flavonoids High dose of extract had a significant relaxation effect on isolated intestinal muscle of rabbits (P <0.01), high dose of valerian triester had some relaxation effect on isolated intestinal muscle (P <0.05) The high dose of triester extract significantly increased the percentage of mice entering the open arms and entering the total number of arms (OE), the activity time in the open arms and the percentage of the total activity time into each arm (OT) (P <0.01). The medium and high dose groups significantly increased the number of mice in the mice and the activity time of the bright box (P <0.01), while the total flavonoids extract dose Mice wear tank and add the number of active time out box no significant effect. Conclusion The total flavonoids of spider incense is the main component of analgesic and gastrointestinal motility. The valerian triester component is the main component of anxiolytic efficacy.