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目的:探讨脂肪来源的干细胞(ADSC)移植对脑缺血大鼠神经轴突生长以及神经胶质酸性蛋白(GFAP)、神经突蛋白(Neuritin)、神经微丝蛋白200(NF-200)表达的影响。方法:54只清洁级成年雄性SD大鼠,随机分为3组:假手术组(Sham组)、模型组(MCAO组)及MCAO+ADSC治疗组(ADSC组),每组18只。采用改良Zea-Longa线栓制法大脑中动脉栓塞(MCAO)模型,ADSC移植前用DAPI标记,ADSC组于造模成功1 d后经侧脑室注射入ADSC(1×106),分别于术后7 d、14 d、28 d观察其恢复情况,并断头取脑,通过免疫荧光、Western blot法检测脑缺血组织中GFAP、Neuritin、NF-200表达情况。结果:ADSC组缺血周边区脑组织中能观察到DAPI染色的阳性细胞;ADSC组与MCAO组相比在各个时间点脑组织中GFAP阳性细胞表达明显降低(P<0.05),神经突蛋白和神经微丝蛋白200表达明显增高(P<0.05)。结论:ADSC移植后可引起脑缺血后期组织中Neuritin、NF-200有效表达,并抑制GFAP阳性细胞增生,促进了神经轴突再生和修复。
OBJECTIVE: To investigate the effects of adipose-derived stem cell (ADSC) transplantation on neurite outgrowth, expression of glial acidic protein (GFAP), neuritin, and neurofilament protein 200 (NF-200) influences. Methods: Fifty-four adult male Sprague Dawley rats were randomly divided into three groups: Sham group, model group (MCAO group) and MCAO + ADSC group (ADSC group), 18 rats in each group. The model of middle cerebral artery occlusion (MCAO) was established by modified Zea-Longa method. DAPI was performed before ADSC transplantation. ADSCs were injected into ADSCs (1 × 106) At 7 d, 14 d and 28 d, the recovery was observed and the brain was decapitated. The expression of GFAP, Neuritin and NF-200 in cerebral ischemia were detected by immunofluorescence and Western blot. Results: Compared with MCAO group, the expression of GFAP positive cells in brain tissue of ADSC group was significantly lower than that of MCAO group (P <0.05), and neurite outgrowth The expression of neurofilament protein 200 was significantly increased (P <0.05). CONCLUSION: After ADSC transplantation, the expression of Neuritin and NF-200 in the late stage of cerebral ischemia can be effectively inhibited, and the proliferation of GFAP positive cells can be inhibited and the axon regeneration and repair can be promoted.