论文部分内容阅读
目的调查四川富顺地区不同程度宫颈病变患者人乳头瘤病毒(HPV)感染状况及基因型分布特点,为该地区HPV感染的预防和宫颈癌防治提供理论依据。方法采用基因芯片技术对宫颈液基细胞技术所获宫颈细胞及手术切除宫颈石蜡组织进行基因分型检查。结果 255例宫颈病变样本中,HPV检出阳性率为63.92%(163/255),其中高危感染占59.22%(151/255),低危感染占20.39%(52/255),单一感染占66.87%(109/163),混合型占33.13%(54/163)。高危型中最常见前6种亚型依次为:HPV16、58、81、52、39和33,低危型中最常见前4种亚型依次为:HPV6、11、43和54。20~29岁与30~39岁年龄段妇女HPV的总感染率明显高于40~49岁年龄段(P<0.05),低危型感染主要集中在20~29岁年龄段,明显高于30~39岁和40~49岁年龄段(P<0.05)。结论富顺地区不同程度宫颈病变患者有较高的HPV感染率,并且高危型感染率较高,具有较强的区域和年龄段分布特点,为本地区性传播疾病及宫颈癌早期诊断、治疗、预后评估及疫苗使用等提供重要的参考依据。
Objective To investigate the status of human papillomavirus (HPV) infection and genotype distribution in patients with cervical lesions of different degrees in Fushun, Sichuan Province, and to provide theoretical basis for the prevention of HPV infection and prevention and treatment of cervical cancer in this area. Methods Genomic microarray technique was used to genotype cervical cells from cervical liquid-based cells and excised cervical paraffin tissue. Results Among 255 cases of cervical lesions, the positive rate of HPV was 63.92% (163/255), of which 59.22% (151/255) were high-risk infections, 20.39% (52/255) were low-risk infections and 66.87 % (109/163), mixed accounted for 33.13% (54/163). The most common top six subtypes of high risk type were HPV16, 58, 81, 52, 39 and 33, the most common first 4 subtypes of low risk type were HPV6, 11, 43 and 54.20-29 The total infection rate of HPV between the age of 30 and 39 was significantly higher than that of the age of 40 to 49 (P <0.05). The low-risk infection mainly concentrated in the age group of 20 to 29 years old, which was significantly higher than that of 30 to 39 years old And 40-49 age group (P <0.05). Conclusions The patients with different degrees of cervical lesions in Fushun have a higher rate of HPV infection and a higher prevalence of high-risk type infection. They have strong regional and age distribution characteristics, which provide a reliable basis for early diagnosis, treatment and prognosis of sexually transmitted diseases and cervical cancer in the region Assessment and vaccine use provide an important reference.