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目的明确乙型肝炎病毒（ＨＢＶ）前Ｃ第１７位和２９位密码子突变体对ｅ抗原合成和分泌的影响。方法用ＰＣＲ 方法扩增ＨＢＶ前Ｃ／Ｃ基因区，克隆后测序及进行序列分析。将ＨＢＶ前Ｃ区Ｔ１８６２／Ａ１８９９位点突变体亚克隆人表达 质粒 ｐＧＥＭＴ中，经体外翻译后，比较野毒株和变异株的表达产物。结果该前 Ｃ区突变株在体外仍能合成 ＨＢＶ ｅ前体 蛋白。结论前 Ｃ区 Ｔ１８６２／Ａ１８９９突变并不能阻断 ＨＢＶ ｅ抗原前体蛋白合成，ｅ抗原前体可能在分泌过程中受阻。
Objective To clarify the effect of pre-C codon 17 and codon 29 mutations on the synthesis and secretion of e antigen in Hepatitis B virus (HBV). Methods The pre-HBV C / C gene region was amplified by PCR, cloned, sequenced and sequenced. The T1862 / A1899 site mutants in the pre-HBV C region were subcloned into the expression vector pGEMT. After translation in vitro, the expression products of wild-type and mutant strains were compared. Results The pre-C mutant still could synthesize HBV e precursor protein in vitro. Conclusion The mutation of T1862 / A1899 in pre-C region can not block the synthesis of HBV e antigen precursor protein. The precursor of e antigen may be blocked in the process of secretion.