论文部分内容阅读
以水稻为模式植物研究了利用碱处理叶片直接用作 PCR扩增的模板 ,并应用于水稻品种种性和纯度鉴定以及分子标记辅助田间育种材料的选择中。该方法具有快速、简便、结果可信度高 ,重复性好 ,对待测植株的损伤小等特点 ,它无需高速冷冻离心机及相应的提取 DNA的试剂 ,故试验成本低 ,检测所需的时间短。尤其是群体较大时 ,此种方法更是显得经济有效 ,利用此种方法在 1 d内可检测 30 0份样品。
Using rice as model plant, the leaves treated with alkali were directly used as templates for PCR amplification and were applied to the identification of rice variety sex and purity and the selection of molecular marker assisted field breeding materials. The method has the advantages of fastness and simplicity, high reliability of results, good repeatability, low damage to the plant to be tested, etc. It does not need a high-speed refrigerated centrifuge and a corresponding reagent for extracting DNA, so the test cost is low, the time required for the test short. Especially when the population is large, this method is even more cost-effective. With this method, 30 0 samples can be detected within 1 day.