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目的研究大气PM2.5对人支气管上皮细胞(16HBE)内钙稳态的影响。方法以50、100μg/ml大气PM2.5对16HBE细胞进行染毒,以生理盐水为对照组,并用终浓度为100μg/ml的钙离子拮抗剂肝素钠进行干预,即设生理盐水对照组、50μg/ml PM2.5组、100μg/ml PM2.5组、肝素钠组、50μg/ml PM2.5+肝素钠组、100μg/ml PM2.5+肝素钠组,分别染毒3、6、24 h后,以流式细胞仪检测细胞内游离钙离子荧光强度。结果 100μg/ml PM2.5染毒16HBE 3 h后,细胞内钙离子荧光强度高于对照组和50μg/ml PM2.5组;当加入胞内游离钙离子拮抗剂肝素钠后,100μg/ml PM2.5+肝素钠组钙离子荧光强度低于100μg/ml PM2.5组。100μg/ml PM2.5染毒16HBE 6 h后,细胞内钙离子荧光强度高于对照组。PM2.5染毒16HBE 24 h后,50μg/ml PM2.5组、100μg/ml PM2.5组、50μg/ml PM2.5+肝素钠组、100μg/ml PM2.5+肝素钠组的细胞内钙离子荧光强度均高于对照组,且100μg/ml PM2.5组细胞内钙离子荧光强度高于50μg/ml PM2.5组。50、100μg/ml PM2.5染毒组随着染毒时间的延长,钙离子荧光强度升高,染毒6、24 h后的钙离子荧光强度均高于染毒3 h后,上述差异均有统计学意义(P<0.05)。结论大气PM2.5可能刺激16HBE细胞释放胞内钙库,导致胞内游离钙离子荧光强度增加。
Objective To investigate the effect of atmospheric PM2.5 on calcium homeostasis in human bronchial epithelial cells (16HBE). Methods 16HBE cells were exposed to 50,100μg / ml atmospheric PM2.5. Normal saline was used as the control group and intervention with heparin sodium, a calcium ion antagonist at a final concentration of 100μg / ml. Normal saline control group, 50μg / ml PM2.5 group, 100μg / ml PM2.5 group, heparin sodium group, 50μg / ml PM2.5 + heparin group, 100μg / ml PM2.5 + heparin group, respectively, exposed to 3,6,24 h After intracellular free calcium fluorescence intensity was detected by flow cytometry. Results After intracellular calcium ion antagonist Heparin sodium 100μg / ml PM2.5 was exposed to 16HBE for 3 h, the intracellular calcium fluorescence intensity was higher than that of the control group and 50μg / ml PM2.5 group. After 100μg / ml PM2 .5 + Heparin sodium group calcium fluorescence intensity lower than 100μg / ml PM2.5 group. The fluorescence intensity of intracellular calcium was higher than that of control after exposure to 16HBE at 100μg / ml PM2.5 for 6 h. PM2.5 exposed to 16HBE for 24 h, 50μg / ml PM2.5 group, 100μg / ml PM2.5 group, 50μg / ml PM2.5 + heparin group, 100μg / ml PM2.5 + heparin group intracellular Calcium ion fluorescence intensity was higher than the control group, and the intracellular calcium fluorescence intensity of 100μg / ml PM2.5 group was higher than that of 50μg / ml PM2.5 group. With the prolongation of exposure time, the fluorescence intensity of calcium ion in 50 and 100μg / ml PM2.5 exposure groups increased, and the fluorescence intensity of calcium ion in 6 and 24 hours after exposure was higher than that in 3 hours after exposure There was statistical significance (P <0.05). Conclusion Atmospheric PM2.5 may stimulate intracellular calcium stores in 16HBE cells, resulting in an increase of intracellular free calcium fluorescence intensity.