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目的:观察白藜芦醇对大鼠酒精性脂肪肝的作用并探讨相关的作用机制。方法:用白酒灌胃诱导建立大鼠酒精性脂肪肝(AFL)模型,分设空白对照组、模型组、硫普罗宁(阳性对照)组、白藜芦醇(62.5、125、250mg/kg)治疗组。白藜芦醇干预9周后,称取大鼠体质量、体脂和肝脏重量计算肝指数与体脂比,分离血清测定血脂、转氨酶、NO和肝组织匀浆测定SOD、MDA指标,病理切片观察肝脏的病理变化。结果:与模型组相比,白藜芦醇(125、250mg/kg)治疗组大鼠的体脂量比、肝指数、丙氨酸转氨酶(ALT)、甘油三脂(TG)、丙二醛(MDA)的含量显著降低,超氧化物歧化酶(SOD)活性显著增加;白藜芦醇(62.5、125、250mg/kg)治疗组天冬氨酸转氨酶(AST)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-D)、一氧化氮(NO)的含量显著降低,低密度脂蛋白胆固醇(HDL-D)含量显著增加,大鼠肝组织脂肪变明显减轻。结论:白藜芦醇具有降低转氨酶、血脂和抗氧化作用,减少肝细胞中的脂质沉积与肝损伤,改善白酒诱导的酒精性脂肪肝。
Objective: To observe the effect of resveratrol on alcoholic fatty liver in rats and to explore the related mechanism. Methods: A rat model of alcoholic fatty liver (AFL) was established by gavage with alcohol and divided into blank control group, model group, tiopronin (positive control group) and resveratrol (62.5,125,250 mg / kg) group. After resveratrol intervention for 9 weeks, the body weight, body fat and liver weight were weighed to calculate the liver index and body fat ratio. Serum lipids, aminotransferase, NO and liver homogenate were measured for SOD and MDA, Observe the pathological changes of the liver. Results: Compared with the model group, the body fat mass ratio, liver index, ALT, TG, MDA of the rats treated with resveratrol (125 and 250 mg / kg) (MDA), and the activity of superoxide dismutase (SOD) increased significantly. The contents of aspartate aminotransferase (AST), total cholesterol (TC) The levels of LDL-D and NO were significantly decreased, the content of HDL-D was significantly increased, and the fatty changes in liver tissue of rats were significantly reduced. CONCLUSION: Resveratrol has the effects of reducing transaminase, lipids and antioxidation, reducing lipid deposition and liver damage in liver cells, and improving alcohol-induced fatty liver induced by liquor.