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目的 :对人乙型肝炎病毒 (HBV)和 (或 )黄曲霉毒素B1(AFB1)引起的树鼠句肝脏病变进行动态比较观察。方法 :成年树鼠句按不同处理分为A(HBV +AFB1)、B(HBV)、C(AFB1)、D(空白对照 ) 4组。全部动物定期肝活检 ,并于 16 0周结束实验时处死所有存活者。各次活检及尸检肝组织均作常规病理组织学检查 ,部分同时作HBsAg及HBxAg免疫组织化学、HBV DNA原位杂交 ,以及PAS、网状纤维染色等检查。结果 :肝组织的炎症及肝细胞增生性改变以A组最明显 ,B组病变发生虽早但发展较慢 ,C组病变发生较迟 ,但发展较快。肝细胞癌 (HCC)仅见于A、C组 ,其发生率及平均发生时间在A、C组分别为 6 7% (14/2 1)和 30 % (3/ 10 ) ,以及 12 0周和 15 3周 (P <0 0 1)。B组虽无HCC发生但可见较大增生或结节形成。D组无明显增生灶或结节。HBV感染标志在整个实验过程中可在A、B组大多数动物的肝组织中检出 ,其阳性检出率波动于 5 0 %～ 90 %。结论 :树鼠句模型适用于对人类肝炎、肝癌等病变的研究。HBV和AFB1具有协同致肝癌作用。
Objective : To compare the dynamic changes of liver lesions in tree and mouse caused by human hepatitis B virus (HBV) and aflatoxin B1 (AFB1). Methods : Adult tree mice were divided into four groups: A (HBV + AFB1), B (HBV), C (AFB1), and D (blank control) groups. All animals had regular liver biopsies and all survivors were sacrificed at the end of the experiment at the end of the 16th week. Liver tissues from each biopsy and post-mortem examinations were subjected to routine histopathological examinations, and some of them were simultaneously examined for HBsAg and HBxAg immunohistochemistry, HBV DNA in situ hybridization, and PAS and reticular fiber staining. Results: Inflammation of hepatic tissue and hepatocyte proliferation were most obvious in group A. Although lesions in group B occurred early but developed slowly, lesions in group C occurred late but developed rapidly. Hepatocellular carcinoma (HCC) was only found in group A and C. The incidence and the average time of onset were 6 7% (14/2 1) and 30% (3/10) in the A and C groups, respectively, and 12 weeks and 15 3 weeks (P < 0 01). Although there was no HCC in group B, large hyperplasia or nodule formation was observed. There was no obvious hyperplasia or nodule in group D. Signs of HBV infection can be detected in the liver tissue of most animals in group A and B during the whole experiment, and the positive detection rate fluctuates from 50% to 90%. Conclusion: The tree mouse model is suitable for the study of human hepatitis, liver cancer and other diseases. HBV and AFB1 have a synergistic effect on liver cancer.