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为了探讨丙型肝炎(丙肝)患者外用血单个核细胞中HCV—RNA检测的临床意义,采用RT-PCR方法检测血清及外周血单个核细胞中HCV-RNA及原位杂交方法检测外周血单个核细胞(PBMC)中正、负链HCV-RNA.结果显示,采用RT-PCR方法检测35/50例(70%)患者血清中HCV-RNA阳性.47/50例(94%)PBMC中HCVRNA阳性,采用原位杂交方法检测,45/50例(90%)PBMC正链HCV-RNA阳性,5/50例(0.1%)负链HCV-RNA阳性,使用正链探针杂交的标本在较多的细胞中出现杂交信号,负链杂交时,仅在少数细胞胞浆中出现杂交信号.采用RT-PCR方法检测外周血单个核细胞中HCVRNA是一种敏感性很高的检查方法.
To investigate the clinical significance of HCV-RNA detection in peripheral blood mononuclear cells of patients with hepatitis C (HCV), the detection of HCV-RNA in peripheral blood mononuclear cells by using RT-PCR and detection of peripheral blood mononuclear cells by in situ hybridization Positive and negative HCV-RNA were detected in PBMCs.The results showed that HCV RNA was positive in sera of 35/50 patients (70%) by RT-PCR and positive in 47/50 (94%) PBMCs, By using in situ hybridization, 45/50 (90%) PBMC positive HCV-RNA positive, 5/50 (0.1%) negative HCV-RNA positive, and positive PCR probes were more positive Hybridization signals appear in the cells, and hybridization signals appear only in the cytoplasm of a few cells when the negative hybridization is carried out. The detection of HCVRNA in peripheral blood mononuclear cells by RT-PCR is a highly sensitive method.