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Δ1-吡咯啉-5-羧酸合成酶(Delta-1-pyrroline-5-carboxylate synthase,P5CS)是脯氨酸代谢途径的关键酶,对植物抵抗逆境胁迫起至关重要的作用。为了探索P5CS在玉米对逆境胁迫的响应,本文对5个玉米P5CS基因家族蛋白的基本理化性质、二级结构预测、亚细胞定位、基因结构、进化关系及保守基序等方面进行初步生物信息分析。结果显示,5个玉米P5CS蛋白中有3个偏酸性,1个显中性,1个偏碱性;2个以无规则卷曲为主要构成元件,3个P5CS蛋白主要以α白螺旋为主要构成元件;不稳定指数分析发现,4个P5CS蛋白为稳定蛋白;疏水性分析表明,所有的P5CS蛋白为亲水性蛋白;玉米P5CS家族内含子最少含有4个内含子,最多的含有19个内含子;亚细胞定位分析表明,P5CS均蛋白定位于细胞基质;进化分析表明,P5CS家族分3个亚家族。
Delta-1-pyrroline-5-carboxylate synthase (P5CS) is a key enzyme in the proline metabolism pathway and plays an important role in the plant against stress. In order to explore the response of P5CS to maize in response to environmental stress, the preliminary bioinformatics analysis of five maize P5CS gene family proteins in terms of their physico-chemical properties, secondary structure prediction, subcellular localization, gene structure, evolutionary relationships and conserved motifs . The results showed that 3 of 5 maize P5CS proteins were acidic, 1 was neutral and 1 was alkaline; 2 were randomly curled and 3 P5CS proteins were mainly composed of α-white helix Element; Four unstable P5CS proteins were found to be stable proteins. Hydrophobicity analysis showed that all the P5CS proteins were hydrophilic proteins. The P5CS family of in maize contained at least 4 introns with a maximum of 19 Intron; subcellular localization analysis showed that P5CS protein localized in the cell matrix; evolutionary analysis showed that P5CS family is divided into three subfamilies.