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目的:进一步研究单结合胆红素(MCB)的水难溶特性及其在病理性胆汁中参与胆红素沉淀和色素性结石形成的作用机制.方法:应用胆红素高效液相色谱分析技术,先从人胆汁中获得结合胆红素,再经分离、制备及纯化得到纯MCB.然后观察其水溶解度并与其它型胆红素比较.结果:在pH7.9,温度37℃接近生理的条件下,MCB的溶解度(558.25±5.96μm)仅为双结合胆红素(DCB)的1/7,比不结合胆红素(UCB)高约44倍并随pH值改变而呈曲线变化.pH7.9时最大;pH4.5时为0;而当pH>9时,反而低于呈离于状态的UCB.结论:MCB虽作为结合型胆红素在胆道生理条件下的不溶性明显高于UCB,但比DCB要低得多;而在胆道病理条件下,其水溶性不但远低于DCB,甚至还不如离子化的UCB,这可能是MCB参与胆红素沉淀及胆色素结石形成的理化基础.
OBJECTIVE: To further investigate the water-insoluble characteristics of single-binding bilirubin (MCB) and its mechanism of involvement in the formation of bilirubin and pigmented stones in pathological bile.Methods: The bilirubin high performance liquid chromatography , First obtained from the bile of human bilirubin, and then separated, prepared and purified pure MCB and then observed its water solubility and compared with other bilirubin.Results: at pH7.9, temperature 37 ℃ close to the physiological The solubility of MCB (558.25 ± 5.96μm) was only 1/7 that of DCB, about 44 times higher than that of UCB, and changed with the change of pH. pH7.9, 0 at pH4, but lower than that of UCB at pH> 9.Conclusion: MCB, as a conjugated bilirubin, is significantly more insoluble in biliary tract physiology than UCB, but much lower than that of DCB. Under the pathological conditions of biliary tract, its water solubility is not only far lower than that of DCB but also ionized UCB, which may be the result of MCB’s involvement in the formation of bilirubin and biliary pigment stones basis.