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目的观察人乳腺癌组织中硒结合蛋白1(SBP1)和凋亡调节蛋白(Caspase-3)的表达,探讨SBP1和Caspase-3在乳腺癌发生、发展过程中的作用及其相互关系。方法用免疫组织化学ABC法检测乳腺癌中SBP1和Caspase-3的表达水平,并与癌旁相对正常组织、乳腺良性病变组织进行比较分析。结果 SBP1、Caspase-3在癌旁相对正常乳腺组织中的阳性表达率为100%、86.6%;在乳腺良性病变组织的阳性表达率为93.3%、73.3%;在乳腺癌组织的阳性表达率为82.1%、57.1%。乳腺癌组织中SBP1和Caspase-3的表达水平均明显低于癌旁相对正常组织和乳腺良性病变组织。乳腺癌组织中SBPl与Caspase-3的表达呈正相关。结论乳腺癌组织中SBP1和Caspase-3的表达下调,提示SBPl可能通过促进肿瘤细胞的凋亡来抑制肿瘤的增殖,为进一步对硒诱导SBP1治疗乳腺癌的作用研究提供基础。
Objective To observe the expression of sepsis binding protein 1 (SBP1) and apoptosis regulatory protein (Caspase-3) in human breast cancer and to explore the roles and relationships of SBP1 and Caspase-3 in the pathogenesis and development of breast cancer. Methods The expression of SBP1 and Caspase-3 in breast cancer was detected by immunohistochemical ABC method. The expression of SBP1 and Caspase-3 in breast cancer tissues were compared with those in normal tissues and benign breast lesions. Results The positive rates of SBP1 and Caspase-3 in the adjacent normal breast tissues were 100% and 86.6%, respectively. The positive rates of SBP1 and Caspase-3 were 93.3% and 73.3% in breast benign lesions. The positive rates of SBP1 and Caspase- 82.1%, 57.1%. The expression levels of SBP1 and Caspase-3 in breast cancer tissues were significantly lower than those in the adjacent normal tissues and benign breast lesions. There was a positive correlation between the expression of SBP1 and Caspase-3 in breast cancer tissues. Conclusions The down-regulation of SBP1 and Caspase-3 expression in breast cancer suggests that SBP1 may inhibit the proliferation of tumor cells by promoting the apoptosis of tumor cells, which may provide a basis for further studies on the effect of selenium-induced SBP1 on breast cancer.