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目的 观察人胎海马结构小白蛋白 (PV)免疫反应性神经元的分布。 方法 取孕龄为 30周的人胎尸体 ,用ABC免疫细胞化学方法显示PV免疫反应性神经元。 结果 海马结构的各区域内均有丰富的PV免疫反应性神经元分布 ,以锥体细胞层最为密集。CA1、CA2、CA3始层PV免疫反应性神经元呈散在分布 ,胞体较小 ,多为三角形或梭形 ,细胞突起 2～ 3个 ,锥体细胞层PV免疫反应性神经元数量最多 ,分布密集 ,细胞形态多样 ,细胞的突起伸向浅层的始层和深层的分子层 ;分子层PV免疫反应性神经元较稀少。门区PV免疫反应性神经元分布密集 ,但细胞分层不明显 ,可见部分细胞的突起伸向齿状回 ;齿状回PV免疫反应性神经元集中分布于颗粒细胞层 ,其余各层有少量散在的PV免疫反应性神经元 ,细胞染色浅淡 ,无明显突起。下托复合体PV免疫反应性神经元主要分布于锥体细胞层 ,始层和分子层较稀少 ,细胞淡染 ,突起不明显。 结论 海马结构的各区域均有丰富的PV免疫反应性神经元分布 ,主要分布于锥体细胞层和齿状回的颗粒层。各区域PV免疫反应性神经元发育成熟的时间可能并不同步 ,CA1～ 3和门区PV免疫反应性神经元发育成熟早于齿状回和下托复合体
Objective To observe the distribution of small albumin (PV) immunoreactive neurons in human fetal hippocampus. Methods The human fetal corpses of 30 weeks of gestational age were used to detect the immunoreactive neurons of PV with ABC immunocytochemistry. Results There were abundant PV immunoreactive neurons in each region of hippocampal formation, with the most dense layer of pyramidal cells. The number of PV immunoreactive neurons in CA1, CA2 and CA3 layer was scattered, the cell body was small, mostly triangular or fusiform, with 2 or 3 protuberances. The number of PV immunoreactive neurons in pyramidal cell layer was the most , The cell morphology is diverse, the protuberances of the cells extend to the beginning and the deep molecular layers of the superficial layers, and the immunoreactive neurons in the molecular layer are scarce. There was a dense distribution of PV immunoreactive neurons in the portal area, but the cell stratification was not obvious, and some of the cells protruded toward the dentate gyrus. The dentate gyrus PV immunoreactive neurons were concentrated in the granular cell layer, Scattered PV immunoreactive neurons, cells stained pale, no significant protrusion. Under the care complex PV immunoreactive neurons are mainly distributed in the pyramidal cell layer, the initial layer and the molecular layer is relatively rare, lightly stained cells, the process is not obvious. Conclusion There are abundant PV immunoreactive neurons in each region of the hippocampal formation, which mainly distribute in the granular layer of pyramidal cells and dentate gyrus. The development and maturation of PV-reactive neurons in all regions may not be synchronized, and the development of PV-reactive neurons in CA1 ~ 3 and portal regions is earlier than that in dentate gyrus and bradycardia complexes