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目的 探讨 ATP生物发光法作为化疗药物敏感性预测方法的可行性。方法 参照 Sevin等 [1 ] 的 2 4孔培养板培养、手工操作检测的方法 ,改进成 96孔微量培养板全自动检测法。通过检测 K 5 6 2细胞对 11种常见抗癌药物的敏感性 ,并与 MTT比色法比较。结果 两者有良好的相关性 (r= 0 .885 ) ,但 ATP法所测得的变异系数明显小于 MTT法(前者为 1.4%～ 13.6 % ,后者为 0～ 2 4.2 % ,P=0 .0 11)。以 ATP标准品作标准曲线 ,当 ATP的浓度在 10 - 6～ 10 - 1 2范围内时 ,ATP的浓度的对数与发光测定值的对数之间有较好的线性关系。ATP测定值与 K5 6 2细胞实际数之间相关性亦很好 (r=0 .994)。结论 ATP生物发光法是一种敏感、可靠的药敏实验方法 ,值得临床进一步研究
Objective To explore the feasibility of ATP bioluminescence as a method for predicting the sensitivity of chemotherapy drugs. Methods According to Sevin et al. [1], the 24-well plate culture and manual detection methods were used to improve the 96-well microplate assay. By detecting the sensitivity of K562 cells to 11 common anti-cancer drugs, it was compared with MTT colorimetry. The results showed good correlation (r = 0.885), but the coefficient of variation measured by the ATP method was significantly less than the MTT method (the former was 1.4% to 13.6%, the latter was 0 to 2 4.2%, P=0. .0 11). Using the ATP standard as a standard curve, when the ATP concentration is in the range of 10 -6 to 10 - 1 2 , there is a good linear relationship between the logarithm of the ATP concentration and the logarithm of the luminescence value. The correlation between ATP measurements and the actual number of K562 cells was also good (r=0.994). Conclusion ATP bioluminescence assay is a sensitive and reliable susceptibility testing method, which is worthy of further clinical study.