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基于TiO2-石墨烯、离子液体和壳聚糖复合膜修饰玻碳电极制备了一种新型的电化学传感器。用循环伏安法研究了血红蛋白在该修饰电极上的直接电化学行为。结果表明,该纳米复合膜能有效地促进血红蛋白在电极上的直接电子转移,保持其生物催化活性。该传感器对H2O2具有良好的催化性能。H2O2的电流响应信号与其浓度在20～860μmol/L范围内呈良好的线性关系,检出限为0.1μmol/L(S/N=3)。传感器具有良好的稳定性和重现性。
A novel electrochemical sensor based on TiO2-graphene, ionic liquid and chitosan composite membrane modified glassy carbon electrode was prepared. Cyclic voltammetry was used to investigate the direct electrochemical behavior of hemoglobin at the modified electrode. The results show that the nanocomposite film can effectively promote the direct electron transfer of hemoglobin on the electrode and maintain its biocatalytic activity. The sensor has good catalytic performance for H2O2. The current response of H2O2 showed a good linear relationship with the concentration of 20 ~ 860μmol / L with the detection limit of 0.1μmol / L (S / N = 3). The sensor has good stability and reproducibility.