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高异黄酮大豆品种中黄68系中国农业科学院作物科学研究所以缺失脂肪氧化酶-2基因(lx-2)的低豆腥味大豆品种中黄18为母本,日本引进的7S蛋白亚基缺失(α’亚基缺失和β亚基含量低)的大豆材料7S3(Karikoi-434)为父本,经有性杂交,采用高效液相色谱(HPLC)技术检测后代籽粒的异黄酮含量,等电聚焦聚丙烯酰胺凝胶电泳(IEFPAGE)技术检测脂肪氧化酶基因缺失,并进行多年生化标记辅助选择而成。该品种2013年通过北京市品种审定委员会审定,其突出特点是高产、稳产、异黄酮含量高(5 135.86 mg·kg-1)、缺失脂肪氧化酶-2基因(lx-2)、商品性好、综合性状优良,属高异黄酮含量低豆腥味的特用大豆新品种。
High Isoflavone Soybean Variety Zhong Huang 68 Department of Crop Science, Chinese Academy of Agricultural Sciences With the deletion of the lipoxygenase-2 gene (lx-2), the low-beany flavor soybean Zhonghuang 18 is the female parent and the Japanese introduced 7S protein subunit is missing (α-subunit deletion and β-subunit content was low) of 7S3 (Karikoi-434) as the male parent, sexual cross, the use of high performance liquid chromatography (HPLC) Focus polyacrylamide gel electrophoresis (IEFPAGE) detection of lipoxygenase gene deletion, and multi-year biomarker-assisted selection from. The variety was approved by the Beijing Variety Approval Committee in 2013. Its outstanding features are high yield, stable yield, high isoflavone content (5 135.86 mg · kg -1), absence of the lipoxygenase-2 gene (lx-2), good commodity , Good comprehensive traits, is a high-isoflavone low-beany flavor special soybean new varieties.